By Alexander Sulakvelidze, Elizabeth Kutter
In line with the emergence of pathogenic micro organism that can not be taken care of with present antibiotics, many researchers are revisiting using bacteriophages, or phages, to struggle multidrug-resistant micro organism. Bacteriophages: Biology and purposes offers exceptional, finished details on bacteriophages and their purposes, reminiscent of phage treatment. It bargains ideas, media, and method interested by separating and dealing with healing phages. photos, line drawings, and electron micrographs of phages also are integrated. With its large process, this booklet is an invaluable reference for microbiologists, hematologists, and infectious illness researchers.
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Extra resources for Bacteriophages: Biology and Applications
Hershey noted that the DNA of phage T2 could be separated from the protein “ghosts” of the phage by in vitro osmotic shock, and he reasoned that this dissociation might be related to some of the long-sought intracellular steps in reproduction of the phage. Most of the famous paper by Hershey and Chase (1952) is devoted to characterizing this dissociation in vitro. They used radioactive-labeled proteins and nucleic acids in the standard parent-to-progeny transfer experiment, but by careful attention to the association of the labeled components with the infectious material, they concluded that DNA in the phage becomes liberated (as determined by slow sedimentation and sensitivity to DNase) when phage adsorb to bacterial debris.
Inhibitors of protein synthesis (amino acid analogues and antibiotics) were observed to block the intracellular development of phage, which was strong evidence that new protein synthesis was needed as part of the process of phage reproduction. , the coat that could be detected antigenically late in the infectious cycle). , deoxycytidylate hydroxymethylase, DNA polymerase, glucosyl transferases, DNases, polynucleotide kinase, and thymidylate synthetase, to mention a few of the many now-known phage-encoded enzymes).
Classification et identification des typhiphages, Comptes rendus Soc Biol Paris, 119, 1270, 1935. , We Can Sleep Later: Alfred D. Hershey and the Origins of Molecular Biology, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 2000. , Molecular Biology of Bacterial Viruses, Freeman, San Francisco, CA, 1963. , Equal transfer of both parental T7 DNA strands to progeny bacteriophage, Nature, 219, 159–160, 1968. , From culture as organism to organism as cell: Historical origins of bacterial genetics, J Hist Biol, 24, 171, 1991.
Bacteriophages: Biology and Applications by Alexander Sulakvelidze, Elizabeth Kutter