By Ashok Agarwal, Sajal Gupta, Rakesh Sharma
This state of the art laboratory handbook contains 20 scientific protocols used day-by-day for the research of the infertile male, offered with effortless to appreciate, step by step technique. The protocols are prepared from regimen to complicated laboratory techniques universal to medical perform, together with computer-assisted semen research, sperm practise for IUI by way of density gradient and swim-up, sperm cryopreservation, and sperm DNA fragmentation try by way of TUNEL technique, between others. The technique in each one protocol follows top perform guidance made clearer through professionally hand-drawn illustrations masking lots of the very important steps and gear. The authors, hailing from the world-renowned Andrology middle at Cleveland hospital, have over 50 years of mixed first-hand event in handling very busy diagnostic and examine amenities in male infertility and andrology. The ebook can be an integral source for millions of laboratory technologists, clinicians and reproductive pros (andrologists, embryologist, etc.) engaged within the prognosis and administration of infertile males round the world.
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Extra info for Andrological Evaluation of Male Infertility: A Laboratory Guide
The reported results should be compared with the information provided by the patient and values recorded in the laboratory during testing. Calculations should be checked. It is a good practice to review other documentations accompanying the test or procedure, such as consent forms, logs, and screening materials. Remedial action should be taken when errors are detected. 2 Computer Data Security The laboratory must, to the best of its ability, protect the conﬁdentiality of patient medical records.
As PMNs are the predominant leukocytes in semen, this method is acceptable in most cases. Fluorescent staining followed by ﬂuorescence microscopy or ﬂow cytometry can be used to differentiate the leukocytes in semen. White blood cells can also be detected from the stained slide used for sperm morphology counts. A differential count of leukocytes can be conducted along enumeration of sperm cells, and the proportion of leukocytes of various types per sperm, along with the sperm concentration, can be used to calculate leukocyte concentration.
1999;14:765–9. 13. Cooper TG, Björndahl L, Vreebrg J, Nieschlag E. Semen analysis and external quality control schemes for semen analysis need global standardization. Int J Androl. 2002;25:306–11. 14. Auger J, Eustach F, Ducot B, Blandin T, Daudin M, Diaz I, El Matribi S, Gony B, Keskes L, Kolbezen M, Lamart A, Lornage J, Nomal N, Pitaval G, Simon O, Virant-Klun I, Spira A, Jouannet P. Intra- and inter-individual variability in human sperm concentration, motility and vitality assessment during a workshop involving ten laboratories.
Andrological Evaluation of Male Infertility: A Laboratory Guide by Ashok Agarwal, Sajal Gupta, Rakesh Sharma