By R. W. Fairbrother (Auth.)
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2 52 A TEXT-BOOK OF BACTERIOLOGY The growth curve of the various bacteria is influenced by a number of factors, the most important of which are the temperature of incubation, the nature, age and amount of the seeded bacterium and the medium employed. The use of the optimum temperature, a satisfactory medium and a fresh actively growing organism considerably shortens both the lag phase and the generation time, while under unfavourable conditions both these phases may be greatly prolonged. Dormancy.
A series of cultures in different media is usually prepared ; an indication of those likely to yield useful results is frequently given by the morphological and staining reactions of the bacterium. , cultures for tubercle bacilli. The various types of media furnish different information ; they may be conveniently grouped into three types : (1) fluid, (2) solid, and (3) those used for biochemical tests. Fluid Media. " (2) The appearance and degree of turbidity in the fluid. , granular, tenacious, heavy, light, readily dispersed, etc.
In hot climates the amount should be increased to 20-25 per cent. High temperatures interfere with its power of solidifying and care is therefore required in sterilization ; steaming on successive days is the usual procedure. Gelatin, if too concentrated, tends to split when inoculated. Agar or Agar-Agar is dried seaweed collected around the shores of China and Japan. , and this constitutes an excellent solid medium, which is known as nutrient agar or simply agar. After the addition of the agar the broth is placed in the steamer for 1 hour to effect solution.
A Text-Book of Bacteriology by R. W. Fairbrother (Auth.)